Isotopic Tracers in Biology. An Introduction to Tracer by Martin D. Kamen

By Martin D. Kamen

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Extra resources for Isotopic Tracers in Biology. An Introduction to Tracer Methodology

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N o visible alteration in the crystals was observed, nor was a n y water-insoluble material formed. T h e crystals were dissolved and purified b y paper and column chromatography. One fraction which behaved in the same m a n n e r as authentic B i 2 was analyzed a n d found t o be identical chemically a n d biologically with vitamin B i 2 . T h e purified material was shown t o account for ^ 8 0 % of both the original radioactivity and bioactivity. Earlier claims for similar high retentions 21 22 during activation of sulfur in cystine have not been confirmed, however.

Over sixty (n,p) reactions have been studied, and t h e radioactive products have been found to be negative β emitters for the most p a r t . I n the region of higher atomic numbers (Z > 35), it is possible for stabilization t o occur either b y negative or positive ß-ray emission because stable isobars occur with some frequency a t higher atomic numbers. For this reason some positron emitters are also noted. 3. Neutron Capture with a-Particle Emission (η,α). This reaction occurs practically only with high-energy neutrons because of the high barrier to α-particle penetration.

T h e vitamin was then exposed to a ther13 2 mal neutron flux of 1 X 10 n e u t r o n s / c m . / s e c . for 7 days a t ~ 8 0 ° C. After irradiation, the sample was allowed t o stand for two months so t h a t all short-lived activities disappeared. N o visible alteration in the crystals was observed, nor was a n y water-insoluble material formed. T h e crystals were dissolved and purified b y paper and column chromatography. One fraction which behaved in the same m a n n e r as authentic B i 2 was analyzed a n d found t o be identical chemically a n d biologically with vitamin B i 2 .

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